Can a virus infect an ISO file?

I doubt that a typical virus would inject itself into an ISO. Note that if your ISO contains a virus already (e.g. the file was infected then put into the ISO) then you'll have issues.

"Not directly, but an ISO is a disc image - it could potentially contain files which could be viruses. Identical to how a ZIP file (or any other archive file format) could contain an infected file.

An ISO is generally less likely to contain malware, as a virus creator could just as easily infect peoples computers with much smaller files (single executables), which they would be more likely to download, but it is possible."

"Whether it can be infected or not doesn't really matter when you can use something like Malwarebytes or AVG to scan it and attempt to heal it if it's infected (be warned if you have any cracked programs or tools for doing such, AVG loves to give false positives on those, but malwarebytes can't scan inside rar files so you almost need both). Just remember to scan it unmounted to check the ISO itself, then mounted to check the files on it.

I personally haven't ever seen an infected ISO, but ANY file could possibly get infected by a virus."